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KMID : 1137020140250040328
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Journal of Gynecologic Oncology
2014 Volume.25 No. 4 p.328 ~ p.333
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Granulocyte-colony stimulating factor decreases the extent of ovarian damage caused by cisplatin in an experimental rat model
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Akdemir Ali
Zeybek Burak
Akman Levent
Ergenoglu Ahment Mete
Yeniel Ahmet Ozgur
Erbas Oytun
Yavasoglu Altug
Terek Mustafa Cosan
Taskiran Dilek
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Abstract
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Objective: To investigate whether granulocyte-colony stimulating factor (G-CSF) can decrease the extent of ovarian follicle loss caused by cisplatin treatment.
Methods: Twenty-one adult female Sprague-Dawley rats were used. Fourteen rats were administered 2 mg/kg/day cisplatin by intraperitoneal injection twice per week for five weeks (total of 20 mg/kg). Half of the rats (n=7) were treated with 1 mL/kg/day physiological saline, and the other half (n=7) were treated with 100 μg/kg/day G-CSF. The remaining rats (n=7, control group) received no therapy. The animals were then euthanized, and both ovaries were obtained from all animals, fixed in 10% formalin, and stored at 4℃ for paraffin sectioning. Blood samples were collected by cardiac puncture and stored at -30℃ for hormone assays.
Results: All follicle counts (primordial, primary, secondary, and tertiary) and serum anti-Mullerian hormone levels were significantly increased in the cisplatin+G-CSF group compared to the cisplatin+physiological saline group.
Conclusion: G-CSF was beneficial in decreasing the severity of follicle loss in an experimental rat model of cisplatin chemotherapy.
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KEYWORD
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Anti-Mullerian hormone, Cisplatin, Granulocyte-colony stimulating factor, Ovarian follicle, Ovary
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